Reconstitution guide
Peptide reconstitution calculator
Answer three things — who you are, which peptide, and how long you have been taking it — and the page builds the rest with your numbers: what to buy, how to mix the powder with the water, which mark to fill the syringe to, and where to give yourself the shot.
Start here
Tell us who you are and what you are taking
Three answers. Everything below then comes with your own numbers: what to buy, how to mix it, and which mark to fill the syringe to.
Step 2
What to have on hand
All of this is sold in any pharmacy, except the vial and the water, which come with your order. If yours are a different size, change them here and the rest of the page follows.
- U-100 insulin syringe, 100 units (1 mL), with a short fine needle (30G or 31G)
- 3 mL syringe with a thicker needle (21G to 25G), just for moving the water into the vial
- Retatrutide, 10 mg
- Bacteriostatic water, 3 mL
Plus the usual: gloves and alcohol wipes.
If your vial or your syringe are different
The most common sizes come preset: 10 mg vial, 3 mL of water and a 100-unit syringe. Change them only if yours are different.
How much peptide powder your vial holds, before any water is added. It is printed on the label and on your order: 5, 10 or 20 mg, for instance. It is a fixed figure — no amount of water changes it.
How much water you will inject into the vial to dissolve the powder. This one is your call: less water leaves the solution more concentrated, more water leaves it more dilute and easier to measure in the syringe. The total amount of peptide does not change — it is simply spread through more or less liquid.
Step 3
How to mix the powder with the water
The peptide arrives as a dry powder and has to be dissolved before you can take any. You do it once per vial and it takes a couple of minutes.
This vial takes 3 mL of water.
Set everything out and clean the table
Gather the vial with the powder, the water that came with your order, new syringes, gloves and alcohol wipes. Work on a clean table wiped down with alcohol.
Note. The water in your order arrives ready to use. It carries a preservative that holds back bacteria, which is why the mix lasts weeks instead of hours.
Clean both rubber caps
Wipe the rubber cap of the powder vial and the one on the water with an alcohol wipe. Let them air-dry for about 30 seconds before any needle goes in. Never blow on them to speed it up.
Draw the water into the big syringe
With a new syringe, draw 3 mL of water. Turn the water bottle over and hold it upside down: it comes out more easily and takes fewer bubbles with it.
Note. This is what the big syringe is for, the 3 mL one with the thicker needle. The insulin one is far too small to move that much water.
Move the water into the powder vial
Tilt the vial a little and let the stream hit the inner wall, never straight onto the powder. Slowly, so it does not foam. Never shake it: that ruins the peptide.
Note. If the vial feels pressurised as the needle goes in, let that off gradually before pushing further. Slow and steady.
Dissolve it by rolling
Roll the vial between your palms in soft circles. Most dissolve in 30 to 60 seconds. It should end up clear, or barely cloudy. If bits are still visible, keep rolling — never shaking.
Write the date and into the fridge
The vial arrives labelled, so all you add is the date you mixed it. Into the fridge straight away, between 2 and 8 °C and away from light. Use it within 60 days: after that it can lose strength.
Note. For long storage, leave the vial with the powder in the freezer and mix it only when you are going to use it. What has been thawed is not frozen again.
Step 4
How much to take
This is your amount for today, based on what you answered above.
Draw 60 units
once a week, with the 100-unit syringe
Retatrutide, average person · 10 mg vial reconstituted in 3 mL of water
Visual guide — draw up to the 60.0 mark on your syringe
Draw 60.0 units of solution into your U-100 insulin syringe and you will have roughly 2 mg of peptide. One unit is 0.01 mL.
Later on, if it stops working for you, it moves to 3 mg. Not on a calendar: if this one is still working, you stay on it.
These ranges come from protocols in common use, not from a prescription. What applies to you is decided by the physician who reviews your consultation. The profile and the syringe units come from that compound's protocol. If yours is not listed, it is because we found no published range for it and we will not invent one: ask about it in the consultation.
Step 5
How and where to give yourself the shot
It goes under the skin, into the layer of fat. There is no need to reach the muscle, which is why the needle is so short.
Where to inject
Three areas. In all three you go under the skin, into the pinch of skin and fat — not the muscle.
Abdomen
About 5 cm from the navel, either side. It is the most used area and the easiest to reach one-handed.
Thigh
The outer or front face, in the middle third. Stay off the inner side.
Upper arm
The outer face, in the top third. It is the hardest to do on yourself: better kept for when someone can help.
Move the spot every week. Always using the same one irritates the skin and in time leaves lumps, which also make it absorb worse.
How to inject
Five steps, with the syringe already filled to the mark given above.
Clean the skin with alcohol
Wipe it and let it air-dry for a few seconds. Going in while the alcohol is still wet stings.
Pinch a little skin
Two fingers, a gentle fold. That lifts the fat away from the muscle, which is where you do not want to end up.
Go straight in, at a right angle
Perpendicular to the skin, in one firm movement, with the insulin syringe and its very thin needle.
Push the plunger slowly
No rush, all the way down. Then let go of the pinch and wait a couple of seconds before pulling the needle out.
Pull the needle out and bin it
It is not used again, not even for the next time. It goes in a sharps box or, failing that, a rigid container with a lid.
Reference
Guide to the units
What each of the units that come up while mixing and drawing actually means.
mg and mcg
1 mg is 1,000 mcg. Peptide is weighed in milligrams, but doses are almost always written in micrograms. Check the unit before you do the arithmetic: getting it wrong there is getting it wrong by a factor of a thousand.
Syringe capacity
Every U-100 insulin syringe shares the same scale: one unit is 0.01 mL. What changes is how far they go — 100u (1.0 mL), 50u (0.5 mL) or 30u (0.3 mL). We recommend always using the 100u.
Concentration
mcg/mL is how much peptide is dissolved in each millilitre of solution. 2,500 mcg/mL means every mL holds 2,500 mcg of peptide.
Volume and dose
The volume you draw depends on your solution's concentration: the higher the concentration, the less volume for the same dose. The calculator handles that conversion for you.
Still not sure about something?
Write to us and we will walk you through your vial. If the question is how much applies to you, that one is answered by the physician who reviews your consultation.
Before you use these numbers
This calculator is a guide and is educational. It is not medical advice, not a prescription, and it does not replace a consultation with a licensed clinician or the care of your own doctor. The ranges come from published protocols in common use; what applies to you — if anything does — is decided by the physician reviewing your case, looking at your history, your medication and your labs.
Statements on this site have not been evaluated by ANMAT, the FDA or any equivalent regulatory authority, and nothing here should be read as claiming that any compound diagnoses, treats, cures, mitigates or prevents any disease. Do not start, change or stop any treatment based on this page.
Do not use it if you are under 18, pregnant or breastfeeding. If any unexpected reaction appears — trouble breathing, swelling, severe pain, vomiting that will not stop — seek medical attention immediately; in an emergency, call your local emergency number or go to an emergency room. A vial is single-use and single-person: needles and syringes are never shared.
The maths depends on the figures you enter being right. Always check the concentration against the vial label and the mark against your own syringe before drawing anything. PeptoClinic is not responsible for use of this information outside individual medical review.
What is published on this site is general information, not a diagnosis and not an instruction for your case. What applies to you comes out of the intake and the physician's review of it.